Solanine Interferes with AKT/p-AKT and PI3K/p-PI3K Pathway to Inhibit HIF and Destroy Cell Energy Metabolism

The purpose of this study was to explore the mechanism Solanine disrupting energy metabolism in human renal cancer ACHN cells and clarify its target. specific method culture cell lines, intervene with high, medium low concentrations. content ATP measured by ELISA method. expression HIF-1α protein PI3K, AKT, p-PI3K, p-AKT PI3K/AKT pathway were detected Western blotting. results showed that compared control group, relative p-PI3K a downward trend increase concentration (P 0.05). In addition, also < According above results, it is suggested can significantly inhibit cells, main which down-regulation HI-1αf downstream PI3K/Akt inhibiting phosphorylation process PI3K/p-PI3K Akt/p-Akt.